A cell is said to be homozygous for a particular gene when identical alleles of the gene are present on both homologous chromosomes.
Humanized mouse models have a wide range of applications in the research fields of AIDS, cancer, infectious disease, and blood disease.
Knock-in (KI) is a technique that utilizes homologous recombination of genes to transfer an exogenous functional gene into a homologous sequence in the cell and genome, and obtain expression well in the cell after gene recombination.
Conditional Knock-out (CKO) is a tissue-specific gene knockout technology achieved by a localized recombination system.
By applying TurboMice™ technology, we can directly screen embryonic stem cells after gene editing in 3-5 days, then construct a tetraploid cell, and get homozygous multi-locus gene-edited mice in 3-5 months after surrogacy by mother mice, which can save 1 year for our clients.
TurboMice™ technology enables precise gene editing of long fragments over 20kb, thereby facilitating the rapid production of complex models such as humanization, conditional knockout (CKO), and large fragment knock-in (KI).